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奥密克戎RBD重组蛋白
义翘神州
40592-V08H121
产品概述
蛋白构建
A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutation G339D, S371L, S373P, S375F, K417N, N440K, G446S, S477N, T478K, E484A, Q493R, G496S, Q498R, N501Y, Y505H) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant B.1.1.529) which emerged in the South Africa.
NP No.
表达宿主
HEK293 Cells
种属
SARS-CoV-2
预测 N 端
Arg 319
分子量
The recombinant SARS-CoV-2 Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.83 kDa.
技术指标
纯度
> 95 % as determined by SDS-PAGE. > 95 % as determined by SEC-HPLC.
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
生物活性
Immobilized human ACE2 protein (mFc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 B.1.1.529 (Omicron) Spike RBD Protein (His Tag)(Cat: 40592-V08H121), the EC50 is 3-10 ng/mL.
应用
Immobilized human ACE2 protein (mFc tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 B.1.1.529 (Omicron) Spike RBD Protein (His Tag)(Cat: 40592-V08H121), the EC50 is 3-10 ng/mL.
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