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新冠德尔塔RBD重组蛋白
义翘神州
40592-V08H115
蛋白构建
A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutations K417N, L452R, T478K) (Arg319-Phe541) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as variant (AY.1/AY.2)) which emerged in the India.
NP No.
表达宿主
HEK293 Cells
种属
SARS-CoV-2
预测 N 端
Arg 319
分子量
The recombinant SARS-CoV-2 Spike RBD consists of 234 amino acids and predicts a molecular mass of 26.60 kDa. As a result of glycosylation, it migrates as an approximately 31.68 kDa band in SDS-PAGE under reducing conditions.
技术指标
纯度
> 95 % as determined by SDS-PAGE.
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
生物活性
Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (K417N, L452R, T478K) Protein (His Tag)(Cat:40592-V08H115), the EC50 of SARS-CoV-2 Spike RBD (K417N, L452R, T478K) Protein (His Tag)(Cat:40592-V08H115) is 10-70 ng/mL.
应用
Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(Cat: 10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike RBD (K417N, L452R, T478K) Protein (His Tag)(Cat:40592-V08H115), the EC50 of SARS-CoV-2 Spike RBD (K417N, L452R, T478K) Protein (His Tag)(Cat:40592-V08H115) is 10-70 ng/mL.
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