技术交流
扫描二维码
新冠德尔塔Spike重组蛋白
义翘神州
40589-V08B16
蛋白构建
A DNA sequence encoding the SARS-CoV-2 Spike S1+S2 (YP_009724390.1, with mutations T19R, G142D, E156G, HR157-158 deletion, L452R, T478K, D614G, P681R, D950N) (Met1-Pro1213) was expressed with a polyhistidine tag at the C-terminus. The mutations were identified in the SARS-CoV-2 variant (known as B.1.617.2) which emerged in the India.
NP No.
表达宿主
Baculovirus-Insect Cells
种属
SARS-CoV-2
预测 N 端
Val 16
分子量
The recombinant SARS-CoV-2 Spike S1+S2 consists of 1207 amino acids and predicts a molecular mass of 134.20 kDa.
技术指标
纯度
> 85 % as determined by SDS-PAGE.
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
生物活性
Immobilized human ACE2 protein (mFc tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S1+S2 (T19R, G142D, E156G, HR157-158 deletion, L452R, T478K, D614G, P681R, D950N) Protein (ECD, His Tag)(Cat:40589-V08B16),the EC50 of SARS-CoV-2 Spike S1+S2 (T19R, G142D, E156G, HR157-158 deletion, L452R, T478K, D614G, P681R, D950N) Protein (ECD, His Tag)(Cat:40589-V08B16) is 200-900 ng/mL.
应用
Immobilized human ACE2 protein (mFc tag)(Cat:10108-H05H) at 2 μg/mL (100 μL/well) can bind SARS-CoV-2 Spike S1+S2 (T19R, G142D, E156G, HR157-158 deletion, L452R, T478K, D614G, P681R, D950N) Protein (ECD, His Tag)(Cat:40589-V08B16),the EC50 of SARS-CoV-2 Spike S1+S2 (T19R, G142D, E156G, HR157-158 deletion, L452R, T478K, D614G, P681R, D950N) Protein (ECD, His Tag)(Cat:40589-V08B16) is 200-900 ng/mL.
| 货号 | 名称 | 品牌 | 购买 |
|---|
粤公网安备 44010602000473号
粤ICP备09063491号
营业执照
