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Nickel Affinity Gel used for the purification of histidine-tagged proteins. The unique, non-charged, hydrophilic linkage of the NTA chelate group to the agarose ensures high selectivity for histidine-tagged proteins and reduced non-specific binding of other proteins. Its high selectivity allows for one-step purification. Affinity gel can be used in either denaturing or non-denaturing conditions.