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Nicotinamide has been used:. as a precursor to study its effect on nicotinamide adenine dinucleotide (NAD) concentration in NAD-deficient mammalian mitochondria. as an inhibitor of sirtuin (SIRT) family of deacetylases in in vitro serine hydroxymethyltransferase 2 (SHMT2) deacetylation assay. to study its effect on ischemia-reperfusion (I-R) injury-induced cell death in pten伪m/m mice
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