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Epidermal Growth Factor, human, animal component free has been used: . as a supplement in in LHC-8 medium to culture liver cell lines . in the fetal bovine serum (FBS)-Dulbecco鈥瞫 modified essential medium (DMEM) /F12 medium for primary culture of human glioma cells. as an additive in the conditional medium of normal fibroblasts (NFs) to study its effect on the migration and invasion of endometrial cancer (EC) cells. as a component in tumorsphere medium
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