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L-Glutamic acid monosodium salt hydrate has been used:. to prepare glutamate stock solution in HEPES-buffered saline (HBS) for glutamate perfusions. as a component in YNG (yeast nitrogen base glutamate) medium. used to treat HT22 hippocampal cells to study its effects on cell viability and to examine the expression of molecules related to apoptotic cell death. as a component of fresh media to culture neurons and to assess glutamate uptake