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Irinotecan hydrochloride has been used:. in combination with 5-fluorouracil for screening growth inhibitory functionality in MDA-MB-231 breast cancer cells.. in chemosensitivity screening of high-grade appendiceal (HGA) and low-grade appendiceal (LGA) organoids.. as a chemotherapeutic agent in the cytotoxicity studies in combination with heat shock proteins inhibitors (HPSC1) in HT29 colon cancer cells.