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Endoproteinase Glu-C from Staphylococcus aureus V8 has been used for:. obtaining proteolytic cleavage fragments of the S-layer protein (from Bacillus stearothermophilus ATCC 12980) to perform affinity studies.. the enzymatic cleavage of native VSTx-3 (voltage sensor toxin 3) peptide for its sequence determination. . limited proteolysis of recombinant purified PimA (phosphatidylinositol mannosyltransferase). . the digestion of glycosylated hemoglobin for isotope dilution liquid chromatography-tandem mass spectrometry analysis.
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