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Phospho(enol)pyruvic acid trisodium salt hydrate has been used in:. several enzyme activity assays, such as PRPP synthetase (PRPPS) assay, ribokinase (RK) assay, adenine phosphoribosyltransferase (APRT) assay. in the reaction mixture for enzyme kinetic assay to determine platelet-derived growth factor receptor 伪 (PDGFRA) kinetic parameters. as a component in buffer B to react with adenosine diphosphate (ADP) to form adenosine triphosphate (ATP) for energy charge measurement