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Silibinin has been used:. to study its effect on gene expression levels of various proteins involved in chromatin regulations of prostate cancer, by real time polymerase chain reaction (RT-PCR). to study its effect on cell proliferation in platelet-derived growth factor (PDGF)-treated human tenon鈥瞫 fibroblasts (HTFs) by investigating the expression of proliferating cell nuclear antigen (PCNA) and by water-soluble tetrazolium salt (WST-1) assay. to examine its effect on gene expression levels of stromelysine 1 (STM1), acetyl hexoseamines and collagen production during skin wound healing. to study its inhibitory effect on Escherichia coli ATP synthase
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