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PR-619 has been used:. as a component of lysis buffer and also as a deubiquitinase inhibitor to treat proteins derived from SILAC-labeled Jurkat cells. as a pan-deubiquitinase inhibitor to study its effect on adenoassociated virus (AAV) transduction. in radioimmunoprecipitation assay buffer (RIPA) buffer used for ubiquitination assays