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Imidazole has been used • in the lysis, wash and elution buffer for the purification of histidine tagged Sonic Hedgehog(shh-N) protein • in elution buffer in stepwise gradient for the purification of histidine tagged aldo keto reductases using nickel affinity chromatography • as a component of homogenization buffer for the purification of phagosomal compartments from dendritic cells
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