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Plumbagin from Plumbago indica has been used: . as a reactive oxygen species agent (ROS) to induce cytotoxicity in mouse embryonic fibroblasts. as an oxidative stress inducer to generate superoxide anion in Saccharomyces cerevisiae. as a reference standard in thin layer chromatography and in spectrophotometric analysis for quantification of plumbagin in Plumbago auriculate samples