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Spin trap reagent used to measure ethanol oxidation to 1-hydroxyethyl radical (HER) which can bind to protein. Has also been used in studies of DNA methylation, iron-mediated polyunsaturated fatty acid peroxidation, and free-radical formation by Alzheimer beta-amyloid peptide. The formation of hydroxyl radical in chondrocytes and cartilage has been detected using POBN.
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