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Individual colonies of transfected cells can be isolated and picked from a plate containing many clones. Isolated clones can be dissociated and passaged free from surrounding cells or pulsed with 50-100 uL growth medium which can be analyzed for secreted products. The sterile cloning cylinders are greased on one end to allow the cylinder to seal to the plate. Cells are then dissociated within the cylinder, resuspeneded, and transferred to a new vessel as a pure colony.These cloning cylinders are sterlized using electron-beam irradiation. The glass cylinders will display a brown tint after being sterlized at the appropriate dosage level.
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