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hIL27
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CY123F0010L等，详见产品详情页
Interleukin-27 (IL-27) is a heterodimeric group 2 receptor ligand molecule that belongs to the IL-6/IL-12 family of long type I cytokines. It is composed of EBI3 (EBV-induced gene 3), a 34 kDa glycoprotein that is related to the p40 subunit of IL-12 and IL-23,and p28, the 28 kDa glycoprotein.that is related to the p35 chain of IL-12. The human EBI3 gene encodes a 229 amino acid (aa) precursor that contains a 20 aa signal peptide and 209 aa mature protein. The mature region contains two potential N-linked glycosylation sites, two fibronectin type III domains, and two pairs of conserved cysteine residues with a WSXWS-like motif that places the molecule in
the hematopoietin receptor family. Although p40, the EBI3 counterpart in IL-12, is known to form homodimers, there is no evidence to date that EBI3 also homodimerizes. Human EBI3 is 61% aa identical to mouse EBI3. The human p28 gene encodes a 243 aa precursor that contains a 28 aa signal sequence and 215 aa mature region. The mature region is characterized by the presence of four alpha -helices, placing it in the IL-6 family of helical cytokines. Human p28 is 74% aa identical to mouse p28. IL-27 is expressed by monocytes, endothelial cells and
dendritic cells. IL-27 binds to and signals through a heterodimeric receptor complex composed of WSX-1 (TCCR) and gp130. Evidence suggests IL-27 interacts only with WSX-1. IL-27 has both anti- and proinflammatory properties. As an anti-inflammatory,IL-27 seems to induce a generalnegative feedback program that limits T and NK-T cell activity. At the onset of infection, IL-27 induces an IL-12 receptor on CD4+ T cells, making them susceptible to subsequent IL-12 activity (and possible
Th1 development).
基本信息：
别名(synonym)：Human IL27; IL-27; IL27; IL-27
subunit alpha; IL27A; IL-27-A; IL27-A; IL27p28;
interleukin 27
来源(Source)：Human embryonic kidney cell, HEK293-derived human IL-27 protein 蛋白结构（Structure): 该蛋白不含标签
基因 ID：Q14213.2 (IL-27EBI3) & AAM34498 (IL-27p28)
氨基酸序列
IL-27 EBI-3(Arg21-Lys229),IL-27p28(Phe29-Pro243)
分子量大小(MW)：56.0 kDa (Monomer)
纯度（Purity）：> 95%，determined by SDS-PAGE
制剂(Formulation)：Solution protein. Dissolved in sterile PBS buffer. This solution can be diluted into other aqueous buffers. Centrifuge the vial prior to opening.
产品数据：
1. SDS-PAGE
4 ug/lane protein was resolved with SDS-PAGE under non-reducing (NR) and reducing (R) conditions and
visualized by Coomassie Blue staining.
2. Gel-filtration
Size-exclusion chromatography of recombinant human IL27 protein (280 nm absorbance)
3. 活性检测（Biological Activity）
Recombinant human IL27 anti-viral activity using HepG2 human hepatocellular carcinoma cells infected with encephalomyocarditis (EMC) virus.
订购信息：
| 货号 | 级别 | 规格 | 牌价/￥ |
| CY123F0010L | RUO | 10μg | 1320 |
| CY123F0100L | RUO | 100μg | 3718 |
| CY123F1000L | RUO | 1000μg | 23980 |
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